Difference between revisions of "Rizzo Lab Research"

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(EGFR and ErbB family)
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Balius, T. E.; Rizzo, R. C. Quantitative Prediction of Fold Resistance for Inhibitors of EGFR. Biochemistry, 2009, 48, 8435-8448. [http://pubs.acs.org/doi/abs/10.1021/bi900729a WEB] [http://rizzo.ams.sunysb.edu/~rizzo/StonyBrook/publications/rizzo019.pdf PDF]
Balius, T. E.; Rizzo, R. C. Quantitative Prediction of Fold Resistance for Inhibitors of EGFR. Biochemistry, 2009, 48, 8435-8448. [http://pubs.acs.org/doi/abs/10.1021/bi900729a WEB] [http://rizzo.ams.sunysb.edu/~rizzo/StonyBrook/publications/rizzo019.pdf PDF]
| [[Image:ErbB_Kinases_research_image1.png|375px|alt=Example alt text| ErbB Kinases ]]
| [[Image:ErbB_Kinases_research_image1.png|thumb|375px|alt=Example alt text| ErbB Kinases ]]

Revision as of 11:53, 3 February 2012




viral membrane fusion inhibitor development.

See the following papers.

Strockbine, B.; Rizzo, R. C. Binding of Anti-Fusion Peptides with HIVgp41 from Molecular Dynamics Simulations: Quantitative Correlation with Experiment. Proteins: Struct. Func. Bioinformatics, 2007, 67, 630-642. PDF

McGillick, B. E.; Balius, T.E.; Mukherjee, S.; Rizzo, R. C. Origins of Resistance to the HIVgp41 Viral Entry Inhibitor T20. Biochemistry, 2010, 49 (17), 3575-3592 doi:10.1021/bi901915g PMID: 20230061 WEB PDF

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EGFR and ErbB family

ErbB (epidermal growth factor receptor) family is a drug target for treating several types of cancers, including lung and breast cancers. ErbB family of receptor tyrosine kinases consists of EGFR, HER2, ErbB3, and ErbB4. Overexpression of EGFR is observed in 62% of NSCLC tumors(nonsmall cell lung cancer) and overexpression of EGFR and HER2 are important prognostic markers for breast cancer. Members of the ErbB family share a similar overall structural architecture comprising: (i) extracellular ligand binding domain, (ii) transmembrane domain, (iii) intracellular juxtamembrane domain, (iv) intracellular tyrosine kinase domain, and (v) C-terminal regulatory region where phosphorylation occurs.We are interested in targeting the tyrosine kinase domain(TKD). Approved small molecules of the TKD domain include erlotinib (Tarceva, OSI Pharmaceuticals), gefitinib (Iressa, AstraZeneca), and lapatinib (Tykerb, Glaxo-SmithKline). A fourth compound called AEE788 (Novartis)is in development. Among them, erlotinib and gefitinib primarily target EGFR and lapatinib is a dual inhibitor of EGFR and ErbB2. Several cancer causing mutations or resistance mutations in EGFR and HER2 have been reported. We are interested in what is the driving force of binding and how these mutations affect binding. Water-mediated interactions seem to be especially important understanding affinity and specificity for this system and of this system.

See the following paper:

Balius, T. E.; Rizzo, R. C. Quantitative Prediction of Fold Resistance for Inhibitors of EGFR. Biochemistry, 2009, 48, 8435-8448. WEB PDF

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ErbB Kinases

Method development

DOCK development

The Rizzo Group co-develops the DOCK program [1] and contributed to the latest to releases:

The release v6.4, greatly improved the sampling behavior with the inclusion of internal energy during growth and minimization. See release notes

The release v6.5 includes a new scoring function termed Footprint similarity score. See release notes

Dock development

Docking Testset Development
To facilitate the improvements to the DOCK codebase and docking protocols, our group has developed a docking testset for pose reproduction.

See Mukherjee, S.; Balius, T.E.; Rizzo, R. C. Docking Validation Resources: Protein Family and Ligand Flexibility Experiments. J. Chem. Inf. Model, 2010, 50, 1986-2000. PDF

To obtain the testset visit Rizzo_Lab_Downloads.

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Docking Scoring Functions

Docking has two tasks: sampling and scoring. To Address scoring . . .

Receptor flexibility is important for docking to address this . . . MultiGrid Docking

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Dock Scoring

Leveraging information from existing inhibitors is a useful paradigm for the discovery of new drugs. One scoring function developed in our group and implemented in DOCK 6.5, footprint similarity (FPS) score, uses an energetic profile or footprint of, for example, a known drug to identify a ligand which make similar interactions and is thus likely to bind. Other ideas for computationally generated references include molecular dynamics weighted ensembles, transition states and more. We are expanding this work on several levels including abstracting this scoring method to a grid-based method. See the following paper for more information on this topic.

Balius, T.E.; Mukherjee, S.; Rizzo, R. C. Implementation and Evaluation of a Docking-Rescoring Method Using Molecular Footprint Comparisons. J. Comput. Chem., 2011, 32, 2273-2289. PDF

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FPS Dock Scoring